Name of the system: Nikon-PicoQuant - FLIM microscope
Location of the instrument: Life Science Building 2nd floor; 2.045 Room
Organisation: Sándor Damjanovich Cell Analysis Core Facility, Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen
Core Facility Homepage: biophys.med.unideb.hu/en/cellularimaging E-mail: dszl@med.unideb.hu
Registration:
- Only authorized and trained Users are allowed to use the Equipment in the Core Facility!
- Users need to attend an instrument specific training program before being allowed to sign up to use the equipment independently.
Online instrument booking: link
Potential use:
- multichannel fluorescence lifetime imaging
- Förster resonance energy transfer (FRET)
- multichannel fluorescence anisotropy imaging
- multicolor fluorescence correlation spectroscopy (FCS, FCCS)
- multichannel fluorescence imaging
Specifications:
HydraHarp 400 Multichannel Picosecond Event Timer & TCSPC Module
The instrument consists of a Nikon Ti-E inverted microscope, with 5 picosecond pulsed lasers (405, 485 nm, 510 nm, 560 nm, 640 nm), as well as time-correlated single photon counting (TCSPC) electronics and a high-performance data processing computer. The instrument is suitable for determining fluorescence lifetime, time-dependent and steady-state anisotropy. From the change of the fluorescence lifetime, the FRET efficiency can be determined, as well as the change in the molecular environment and interactions of the fluorophores. The microscope is also suitable for measuring multi-channel fluorescence autocorrelation and cross-correlation (FCS, FCCS), from which molecular mobility (e.g. DNA binding of transcription factors) and co-mobility (stable interaction of proteins) can be deduced.
Screenshot: