Excel spreadsheet for calculating FRET efficiency from ensemble data (medians or trimmed means of cell or pixel populations), with pre-loaded spectral data for the most commong FRET fluorophores


Research - Software - FRETcalc.xlsx
This excel spreadsheet can be used to easily calculate FRET efficiencies medians or (trimmed) means of cell or pixel populations, when using the intensity based (ratiometric, or, in other nomenclature, the "three-cube" method).

The spreadsheet is pre-loaded with spectral data of the most popular fluorophores and offers the selection of these fluorophores and the applied laser lines from pull-down menus. Then it leads the user through the input of necessary measurement data in logical order, to finally calculate the FRET efficiency.

For theroretical background, please see:

Flow Cytometric FRET Analysis of Protein Interactions
in: Flow Cytometry Protocols, 4th edition, eds: Teresa S. Hawley and Robert G. Hawley

If you find this spreadheet useful in your work, please consider citing the original publications for which the speradsheet was prepared:

Methods Mol Biol. 2018;1678:393-419. doi: 10.1007/978-1-4939-7346-0_17.


Methods Mol Biol. 2011;699:371-92. doi: 10.1007/978-1-61737-950-5_18.

If you have any suggestions for modifications, or other questions, please do not hesitate to write to us:

László Ujlaky-Nagy and György Vereb




Last update: 2023. 11. 21. 08:47